SEM functions by using a focussed beam of electrons to investigate the sample surface through a series of steps:
Scanning electron microscopy when used correctly is a versatile and indispensable tool with a wide range of applications across industries.
SEM allows microbiologists to visualise and study microorganisms with exception detail. This can provide insight into microbial morphology and interactions, aiding in understanding behaviour and adaptations.
This technique can also provide intricate views of biological specimens, allowing for examination of cellular morphology and interactions at the nanoscale. This has contributed significantly to several fields, including regenerative medicine, drug development, and medical diagnostics.
To interpret SEM results, the generated images must be analysed, identifying surface details, morphology and features. During this analysis, consider the scale for size context. The sample’s composition can then be determined using energy dispersive spectroscopy (EDS) data, and any unique features or patterns identified. Furthermore, evaluate the surface topography, particle size and distribution, taking defects and anomalies into account. If necessary, quantitative measurements using the SEM software can be performed, allowing for comparison of results to research objectives and complementary secondary techniques can be performed.